Table 2 Oligonucleotide primers with restriction sites
From: Promoters from the itaconate cluster of Ustilago maydis are induced by nitrogen depletion
| Plasmid | Primers/specifications | Restriction enzymes |
|---|---|---|
| pUMa43 | P otef -gfp-Tnos; ori ColE1; ampR; U. maydis ip R-locus | |
| P tad1 -gfp |
fwd: CTCGGTACCTTCGACTTGGTGGATACTGCGGCTGTTG rev: GTCGGTCTTGACTCGACCCAGCTCACCGGATCCGTA | KpnI/BamHI |
| P itp1 -gfp |
fwd: TCGAAATTCGAGCTCGGTACCGACCTGACAGAAGAGATAG rev: AGCTCCTCGCCCTTGCTCACCATGGTTCGACTTGGTGGATACTG | KpnI/NcoI |
| P adi1 -gfp |
fwd: TCGAAATTCGAGCTCGGTACCTCAGCCGATAGGTTTCAC rev: AGCTCCTCGCCCTTGCTCACCATGGTGAGCTGGGTCGAGTC | KpnI/NcoI |
| P mtt1 -gfp |
fwd: TCGAAATTCGAGCTCGGTACCGTTTACCGCACGCTGTA rev: AGCTCCTCGCCCTTGCTCACCATGGTGGATGACGAATCTCAAG | KpnI/NcoI |