Truncation of the transcriptional repressor protein Cre1 in Trichoderma reesei Rut-C30 turns it into an activator

Table 1 Primers used for strain construction in this study

Primer name	Sequence 5′–3′	References
5′cre1_NotI fwd	GCGGCCGCTGGAGGTGACGAGAAGAAAAATTCAGG	This study
5′cre1_XmaI rev	CCCGGGAGTCAAAAAGCAAGTACGCGACGTTG	This study
hph_XmaI_BamHI	CCCGGGTTGGATCCAGGGAGACGAGGTTGTGATGAATAC	This study
hph_SpeI rev	ACTAGTAAGTAGCACCGCTGTCGTCTG	This study
5Pcre1_NotI fwd	GCGGCCGCAGCCAAGACTCAGCATAAAGAGGTTG	This study
5Pcre1_XmaI rev	CCCGGGAGGTACCAAACAAAGCGAGCAAGTAC	This study
ptef_BspEI fwd	TCCGGATGTGTGACAGCTCGCGCAG	This study
ptef_NdeI rev	CATATGTGACGGTTTGTGTGATGTAGCGTG	This study
cre1-96_NdeI fwd	CATATGATGCAACGAGCACAGTCTGCC	This study
Cre1-96_BamHI rev	GGATCCTTAGAAAAAAAAGCAGGTAATGGAGGTGC	This study
cre1-96_BspEI fwd	TCCGGAATGCAACGAGCACAGTCTGCC	This study
cre1-96-TAA_NdeI rev	CATATGGAAAAAAAAGCAGGTAATGGAGGTGC	This study
linker_NheI rev	GCTAGCGCGGGGGGCGCAC	This study
linker_NdeI fwd	CATATGCACAACATGGTCAAGCAGAAGC	This study
YFP_NheI fwd	GCTAGCATGGTCAGCAAGGGCGAGG	This study
YFP_BamHI rev	GGATCCCTTGTACAGCTCGTCCATGCCG	This study

ISSN: 2054-3085