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Table 2 Efficiencies of correct replacements of target genes with the UDH expression cassette in multiplexed CRISPR/Cas9 using RNP complexes

From: Development of microtiter plate scale CRISPR/Cas9 transformation method for Aspergillus niger based on in vitro assembled ribonucleoprotein complexes

Target genes gRNA Colonies screened Correct ΔgaaA Correct Δ39114 Correct ΔgaaX All correct Targeting efficiency (%)
gaaA gERA-009 2 2 – – 2 100
gERA-010 5 5 – – 5 100
gERA-009 + gERA-010 5 5 – – 5 100
gaaA + 39114 gERA-009 + gERA-015 4 3 1 – 1 25
gERA-010 + gERA-016 7 1 2 – 0 0
gERA-009 + gERA-015+ 8 1 3 – 1 13
gERA-010 + gERA-016       
gaaA + 39114 + gaaX gERA-001 + gERA-009 + gERA-015 6 3 1 2 0 0
gERA-002 + gERA-010 + gERA-016 6 1 1 4 1 17
gERA-001 + gERA-009 + gERA-015 + gERA-002 + gERA-010 + gERA-016 5 2 2 2 0 0
  1. Two different crRNAs for each of the target genes were designed and tested in different combinations
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Fungal Biology and Biotechnology

ISSN: 2054-3085

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