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Table 3 A. niger colony expression levels of crh genes in different zones grown on xylose

From: Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

  crhA crhB crhC crhD crhE crhF crhG
N402 zone 1 3.49 6.18 19.90 7.12 5.20 4.99 0.85
N402 zone 3 3.18 8.50 19.23 7.46 4.59 7.03 0.93
N402 zone 5 2.39 39.65 8.63 10.84 2.11 11.50 1.25
  1. Seven-day old sandwiched colonies grown on xylose were used for RNA isolation and subsequent microarray analysis [29]. Distinct zones of the mycelium (zone 1, 3, and 5) harvested form the colony. Zone 1 represents the oldest or central part of the colony. Zone 5 represents the youngest or peripheral part of the colony, whereas zone 3 represents the intermediate zone. Expression levels are represented as percentage of actin expression