Table 4 Gene knockout efficiency per target in Aspergillus niger
| Gene target | Knockout efficiency (%)a | ∆kusA protoplast survival (%) knockout repair DNA fragment (with/without) |
|---|---|---|
| brnA | 100% (9/9) | 0% (17/0) |
| crhA | 100% (5/5) | 6.9% (72/5) |
| crhB | 100% (5/5) | 0% (12/0) |
| crhC | 83% (5/6) | 82% (56/46) |
| crhD | 66.7% (2/3) | 0% (6/0) |
| crhE | 80% (4/5) | 87.5% (280/245) |
| crhF | 100% (2/2) | 0% (2/0) |
| crhG | 100% (4/4) | 0% (18/0) |