Selection markers for transformation of the sequenced reference monokaryon Okayama 7/#130 and homokaryon AmutBmut of Coprinopsis cinerea

Table 4 Transformations of C. cinerea PG78 (trp1.1,1.6, pab1-1) with either trp1⁺ plasmid pBD5 or pab1⁺ vector pPAB1-2 alone or, using same batches of protoplasts, in combination with other non-directly selectable vectors

Plasmid(s)	Transformants collected on							Total transformants	Ratio of clones
Plasmid(s)	1st day	2nd day	3rd day	4th day	5th day	6th day	7th day	Total transformants	Ratio of clones
Experiment 1: trp1⁺ selection
pBD5	–	–	–	21	26	14	6	67	1.0
pBD5 + pYSK7	10	16	31	31	14	4	0	106	1.6
pBD5 + pDB3	2	4	0	50	69	15	12	152	2.3
pBD5 + pCcAde8	–	–	–	45	67	20	16	148	2.2
pab1⁺ selection
pPAB1-2	40	18	14	8	–	–	–	80	1.0
pPAB1-2 + pYSK7	40	13	40	11	–	–	-	104	1.3
pPAB1-2 + pDB3	53	11	31	8	4	–	–	107	1.3
pPAB1-2 + pBD5	14	6	19	13	15	3	–	70	0.9
pPAB1-2 + pCcAde8	59	32	49	9	3	–	–	152	1.9
Experiment 2: trp1⁺ selection
pBD5	20	21	20	15	7	–	–	83	1.0
pBD5 + pYSK7	26	42	31	13	13	–	–	125	1.5
pBD5 + pDB3	34	38	29	13	7	–	–	121	1.5
pBD5 + pCcAde8	18	27	49	16	12	–	–	122	1.5
pab1⁺ selection
pPAB1-2	25	29	50	19	13		–	136	1
pPAB1-2 + pYSK7	40	19	37	37	12	–	–	145	1.1
pPAB1-2 + pDB3	33	46	33	26	17	–	–	155	1.1
pPAB1-2 + pBD5	7	30	37	25	18	–	–	117	0.9
pPAB1-2 + pCcAde8	37	32	54	38	18	–	–	177	1.3

ISSN: 2054-3085